Peer-reviewed articles and reviews
Markus LMD*, Sharon I*, Munro K, Grogg M, Hilvert D, Strauss M§, Schmeing TM§. Structure and function of a hexameric cyanophycin synthetase 2. Protein Science 2023 May 24:e4685. doi: 10.1002/pro.4685. Online ahead of print. PMID: 37222490
Sharon I, Hilvert D, Schmeing TM. Cyanophycin and its biosynthesis: not hot but very cool. Natural Products Reports 2023 May 26. doi: 10.1039/d2np00092j. Online ahead of print. PMID: 37231979
Naineni SK, Cencic R, Robert F, Brown L, Haque M, Scott-Talib J, Sénéchal P, Schmeing TM, Porco JA Jr, Pelletier J. Exploring the targeting spectrum of rocaglates among eIF4A homologs. RNA 2023 Jun; 29(6):826-835. PMID: 36882295
Sharon I, Schmeing TM. Bioinformatics of cyanophycin metabolism genes and characterization of promiscuous isoaspartyl dipeptidases that catalyze the final step of cyanophycin degradation. Scientific Reports 2023 13:8314. PMID: 37221236
Swain K, Sharon I, Blackson W, Tekel S, Schmeing TM, Nielsen DR, Nannenga B. Heterologous production of cyanophycin with Tatumella morbirosei cyanophycin synthetase. Biochemical Engineering Journal 2023 195(108916). doi.org/10.1016/j.bej.2023.108916
Sharon I*, McKay G*, Nguyen D§, Schmeing TM§. Discovery of cyanophycin dipeptide hydrolase enzymes suggests widespread utility of the natural biopolymer cyanophycin. Proceedings of the National Academy of Sciences USA 2023 120 (8), e2216547120. PMID: 36800389
Burns L, Giannakopoulou N, Zhu L, Khan RH, Bekal S, Schmeing TM, Gruenheid S. The bacterial virulence factor NleA undergoes host-mediated O-linked glycosylation. Molecular Microbiology. 2023 119:161-173. PMID: 36196760
Girgis S, Xu Z, Oikonomopoulos S, Fedorova A, Schmeing TM, Götte M, Sonenberg M, Baranov PV, Ragoussis I, Hobman T, Pelletier J. SARS-CoV-2 Defective Interfering Particles. Communications Biology 2022 Oct 27;5(1):1140. PMID: 36302891
Sharon I, Grogg M, Hilvert D, Schmeing TM. The structure of cyanophycinase in complex with a cyanophycin degradation intermediate. Biochimica et Biophysica Acta - General Subjects 2022 November 1866(11), 130217. PMID: 35905922
Patteson JB*, Fortinez CM*, Putz AT, Rodriguez-Rivas J, Bryant LH, Adhikari K, Weigt M, Schmeing TM§, Li B§. Structure and function of a dehydrating condensation domain in nonribosomal peptide synthesis. Journal of the American Chemical Society. 2022 Aug 10;144(31):14057-14070. PMID: 35895935
Sharon I, Pinus S, Grogg M, Moitessier N, Hilvert D, Schmeing TM. A cryptic third active site in cyanophycin synthetase creates primers for polymerization. Nature Communications 2022 Jul 7; 13:3923. PMID: 35798723
Sharon I, Grogg M, Hilvert D, Schmeing TM. Structure and function of the β-Asp-Arg polymerase cyanophycin synthetase 2. ACS Chemical Biology 2022, 17, 3, 680-700. PMID: 35179888
Fortinez CM, Bloudoff K, Sharon I, Harrigan C, Strauss M, Schmeing TM. Structures and function of a nonribosomal peptide synthetase module – tailoring oxidase complex. Nature Communications 2022 Jan 27; 13:548. PMID: 35087027
Stille J, Tjutrins J, Wang G, Venegas FA, Hennecker C, Rueda AM, Sharon I, Miron CE, Pinus S, Labarre A, Plescia J, Patrascu MB, Zhang XC, Wahba AS, Vlaho D, Huot M, Schmeing TM, Mittermaier AK, Moitessier N. Design, synthesis and biological evaluation of novel SARS-CoV-2 3CLpro covalent inhibitors. European Journal of Medicinal Chemistry 2022 Feb 5; 229(114046). PMID: 34995923
Sharon I, Haque AH, Grogg M, Lahiri I, Seebach D, Leschziner AE, Hilvert D, Schmeing TM. Structures and function of the amino acid polymerase cyanophycin synthetase. Nature Chemical Biology 2021 Oct; 17, 1101–1110. PMID: 34385683
Sullivan JR, Lupien A, Kalthoff E, Hamela C, Taylor L, Munro KA, Schmeing TM, Kremer L, Behr MA. Efficacy of epetraborole against Mycobacterium abscessus is increased with norvaline. PLOS Pathogens 2021 Oct 12;17(10):e1009965. PMID: 34637487
Gonçalves CC, Sharon I, Schmeing TM, Ramos CHI, Young JC. The chaperone HSPB1 prepares protein aggregates for resolubilization by HSP70. Scientific Reports 2021 Aug 24;11(1):17139. PMID: 34429462
Golkar T, Bassenden AV, Arya DP, Schmeing TM, Berghuis AM. Structural basis for plazomicin antibiotic action and resistance. Communications Biology. 2021 Jun 11;4(1):729. PMID: 34117352
Alonzo DA, Schmeing TM. Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations. Protein Science 2020 Dec;29(12):2316-2347. PMID 33073901
Robert F, Cencic R, Cai R, Schmeing TM, Pelletier J. RNA-tethering assay and eIF4G:eIF4A obligate dimer design uncovers multiple eIF4F functional complexes. Nucleic Acids Research. 2020 Sep 4;48(15):8562-8575.; PMID 32749456
Alonzo DA*, Chiche-Lapierre C*, Tarry MJ, Wang J, Schmeing TM. Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis. Nature Chemical Biology. 2020 May; 16(5):493-496. PMID: 32066969
*Reimer JM, *Eivaskhani M, Harb I, Guarné A, Weigt M, Schmeing TM. Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility. Science. 2019 Nov 8; 366(6466). PMID: 31699907
Tarry MJ, Harmel C, Taylor J, Marczynski G, Schmeing TM. Structures of GapR reveal a central channel which could accommodate B-DNA. Sci Reports. 2019 Nov 13; 16679 PMID: 31723182
Layoun A, Goldberg AA, Baig A, Eng A, Attias O, Nelson K, Carella A, Amberber N, Fielhaber J, Joung KB, Schmeing TM, Han YS, Divangahi M, Downey J, Roux PP, Kristof AS. Regulation of PKC delta nuclear import and apoptosis by mechanistic target of rapamycin complex-1. Sci Reports. 2019 Nov 26; 9(1) 17620. PMID: 31772273
*Huguenin-Dezot N, *Alonzo DA, Heberlig GW, Mahesh M, Nguyen DP, Dornan MH, Boddy CN, **Schmeing TM, **Chin JW. Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid. Nature. 2019 Jan; 565(7737): 112-117. PMID: 30542153
Reimer JM, Harb I, Ovchinnikova OG, Jiang J, Whitfield C, Schmeing TM. Structural insight into a novel formyltransferase and evolution to a nonribosomal peptide synthetase tailoring domain. ACS Chem Bio. 2018 Nov 16;13(11):3161-3172. PMID: 30346688
Reimer JM, Haque AS, Tarry MJ, Schmeing TM. Piecing together nonribosomal peptide synthesis. Current Opinions in Structural Biology. 2018 Feb 11;49. PMID: 29444491
Langlais D, Cencic R, Moradin N, Kennedy JM, Ayi K, Tarry MJ, Schmeing M, Kain KC, Porco JA, Pelletier J, Gros P. Rocaglates as dual-targeting agents for severe malaria. Proc Nat Acad USA. 2018 Mar 6;115(10):E2366-E2375. PMID: 29463745
Bloudoff K, Schmeing TM. Structural and functional aspects of the nonribosomal peptide synthetase condensation domain superfamily: discovery, dissection and diversity. Biochim Biophys Acta. 2017 Nov;1865:1587-1604. PMID: 285256268
Tarry MJ, Haque AS, Bui KH, Schmeing TM. X-ray crystallography and electron microscopy of cross- and multi-module nonribosomal peptide synthetase proteins reveal a flexible architecture. Structure. 2017 May 2;25(5):783-793. PMID: 28434915
Bloudoff K, Fage CD, Marahiel MA, Schmeing TM. Structural and mutational analysis of the nonribosomal peptide synthetase heterocyclization domain provides insight into catalysis. Proc Natl Acad Sci USA. 2017 Jan 3;114(1):95-100. PMID: 27994138
Schmeing TM. Visualizing A Natural Antibiotic Nanofactory. Clin Invest Med. 2016 Dec 1;39(6):E220-E226. PMID: 27917781
Reimer JM, Aloise MN, Powell HR and Schmeing TM. Manipulation of an existing crystal form unexpectedly results in interwoven packing networks with pseudo-translational symmetry. Acta Crystallogr Sect D. 2016; 1;72 (Pt 10):1130-1136. PMID: 27710934
Bloudoff K, Alonzo DA, Schmeing TM. Chemical Probes Allow Structural Insight into the Condensation Reaction of Nonribosomal Peptide Synthetases. Cell Chem Biol. 2016 Mar 17;23(3). PMID: 26991102
Alonzo DA, Schmeing TM. Translation: Ribosomes make sweeping arrests. Nat Chem Biol. 2016 Feb 16;12(3). PMID: 26881763
Reimer JM, Aloise MN, Harrison PM, Schmeing TM. Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase. Nature. 2016 Jan 14;529(7585). PMID: 26762462
Argyropoulos P, Bergeret F, Pardin C, Reimer JM, Pinto A, Boddy CN, Schmeing TM. Towards a characterization of the structural determinants of specificity in the macrocyclizing thioesterase for deoxyerythronolide B biosynthesis. Biochim Biophys Acta. 2016 Mar;1860(3). PMID: 26592346
Alonzo DA, Magarvey NA, Schmeing TM. Characterization of cereulide synthetase, a toxin-producing macromolecular machine. PLoS One. 2015 Jun 4;10(6). PMID: 26042597
Tarry MJ, Schmeing TM. Specific disulfide cross-linking to constrict the mobile carrier domain of nonribosomal peptide synthetases. Protein Eng Des Sel. 2015 Jun;28(6):163-70. PMID: 25713404
Cencic R, Miura H, Malina A, Robert F, Ethier S, Schmeing TM, Dostie J, Pelletier J. Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage. PLoS One, 2014 Oct 2;9(10). PMID: 25275497
Bloudoff K, Rodionov D, Schmeing TM. Crystal Structures of the First Condensation Domain of CDA Synthetase Suggest Conformational Changes during the Synthetic Cycle of Nonribosomal Peptide Synthetases. J Mol Biol. 2013 Sep 9;425(17):3137-50. PMID: 23756159
Bloudoff K, Schmeing TM. Crystallization and preliminary crystallographic analysis of the first condensation domain of viomycin synthetase. Acta Crystallogr Sect F. 2013 Apr 1;69(Pt 4):412-5. PMID: 23545648
Schmeing TM*, Voorhees RM*, Kelley AC, Ramakrishnan V. How mutations in tRNA distant from the anticodon affect the fidelity of decoding. Nat Struct Mol Biol. 2011, Apr; 18(4):432-6. PMID: 21378964
Voorhees RM*, Schmeing TM*, Kelley AC, Ramakrishnan V. The mechanism for activation of GTP hydrolysis on the ribosome. Science. 2010, Nov 5; 330(6005):835-8. PMID: 21051640
Schmeing TM, Ramakrishnan, V. What recent ribosome structures have revealed about the mechanism of translation. Nature. 2009, Oct 29; 461(7268):1234-1242. PMID: 19838167
Passmore LA, Schmeing TM, Maag D, Applefield A, Acker MG, Algire MA , Lorsch JR, and Ramakrishnan V. The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome. Mol Cell. 2007 Apr 13;26(1):41-50. PMID: 17434125
Schmeing TM, Huang KS, Strobel SA, Steitz TA. An induced fit mechanism to promote peptide bond formation and exclude hydrolysis of peptidyl-tRNA. Nature. 2005 Nov 24;438(7067):520-4. PMID: 16306996
Schmeing TM, Huang KS, Kitchen DE, Strobel SA, Steitz TA. Structural insights into the roles of the 2' hydroxyl of the peptidyl-tRNA and water in the peptidyl transferase reaction. Mol Cell. 2005 Nov 11;20(3):437-48. PMID: 16285925
Schmeing TM, Moore PB, Steitz TA. Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit. RNA. 2003, Nov; 9(11):1345-52. PMID: 14561884
Hansen JL, Schmeing TM, Moore PB, Steitz TA. Structural insights into peptide bond formation. Proc Natl Acad Sci USA. 2002 Sep 3;99(18):11670-5. PMID: 12185246
Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA. A pre - translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits. Nat Struct Biol. 2002 Mar;9(3):225-30. PMID: 11828326
Hansen JL, Schmeing TM, Klein DJ, Ippolito JA, Nissen P, Ban N, Moore PB, Steitz TA (2001) "Progress towards an understanding of the structure and enzymatic mechanism of the large ribosomal subunit." Cold Spring Harbor Symposia on Quantitative Biology. 2001; 66:33-42. PMID: 12762006
Klein DJ*, Schmeing TM*, Moore PB, Steitz TA. The kink-turn: a new RNA secondary structure motif. EMBO J. 2001 Aug 1;20(15):4214-21. PMID: 11483524
Chu LL, Rutteman GR, Kong JM, Ghahremani M, Schmeing M, Misdorp W, van Garderen E, Pelletier J. Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia. Breast Cancer Res Treat. 1998 Jul;50(1):11-25. PMID: 9802616